To ascertain that PAO1 was able to form the biofilm, typical of CF lung colonization, we injected about 100 cfu of PAO1 in the hindbrain ventricle of 24 hpf zebrafish embryos, as reported by Rocker and colleagues24, and observed bacterial colonization by time laps analyses at confocal microscopy (Suppl. Here, SMOX is linked to cystic fibrosis.