TFRC and hepatocellular carcinoma: To analyze whether TFR1, at least in part, mediates the effects of miR-148a on HCC proliferation, we transiently co-transfected HepG2 and Huh7 cells with pcDNA-TFR1, a mammalian expression plasmid encoding the complete human TFR1 CDS, and the miR-148a mimic (TFR1 + miR-148a mimic).