In order to explore the functional role of MAPK4 on MM, plasmids highly expressed MAPK4 (MAPK4) and knockdown ofMAPK4 by shRNA (sh-MAPK4) were constructed and the efficiency of both plasmids was tested by qRT-PCR, which showed a significant increment for MAPK4 (P < 0.001) and reduction for sh-MAPK4 (P < 0.0001), respectively (Fig. 5c). This evidence concerns the gene MAPK4 and Miyoshi myopathy.