In order to clarify the mechanism of miR-154-5p and miR-376b-3p regulating the malignant biological behavior of tumor by regulating FOXP2, after overexpression and silencing of miR-154-5p in U87 and U251 cells, by using qRT-PCR and the Western blot methods to detect the expression of FOXP2, the results showed that compared with the pre-miR-154-5p-NC group, the expression of FOXP2 was significantly decreased in pre-miR-154-5p group (P < 0.01). Here, FOXP2 is linked to neoplasm.