Compared with group ex-NC, p-PI3K/PI3K and p-Akt/Akt increased significantly in group ex-KDM5B; compared with group sh-NC cells, p-PI3K/PI3K, and p-Akt/Akt decreased significantly in sh-KDM5B group, the differences were statistically significant (P < 0.01) (Fig. 5e), it is concluded that KDM5B can regulate the biological behavior of glioma cells by activating the PI3K/Akt pathway. Here, AKT1 is linked to central nervous system cancer.