The experiment shows that the overexpression of miR-154-5p or miR-376b-3 inhibits proliferation, migration, and invasion of malignant glioma and promotes apoptosis; the overexpression of FOXP2 promotes proliferation, migration, and invasion of malignant glioma and inhibits apoptosis; only the overexpression of 3 ‘UTR wild type FOXP2 can reverse the inhibition of proliferation, migration, and invasion of glioma cells mediated by overexpressing miR-154-5p or miR-376b-3p, and also promote apoptosis of the glioma cells. The gene discussed is FOXP2; the disease is malignant glioma.