This study illustrated that the overexpression of KDM5B increases p-PI3K/PI3K, p-Akt/Akt, and also increases the activity of PI3K/Akt pathway, promotes the proliferation, migration, and invasion of glioma cells, and inhibits apoptosis; The expression of silent KDM5B inhibits p-PI3K/PI3K, p-Akt/Akt, namely, inhibits the activity of PI3K/Akt pathway, and also inhibits the proliferation, migration, and invasion of glioma cells, and promotes apoptosis. Here, KDM5B is linked to glioma.