As FAs are important sub-cellular structure mediating cell adhesion to ECM in tumor spreading [59, 60], we then determined by immunofluorescence assays that FAs formation prompted by E2 and G1 is prevented using the GPER antagonist G-15 (Fig. 4a-c), thus suggesting the involvement of GPER in the above mentioned response observed in MDA-MB 231 cells. This evidence concerns the gene GPER1 and neoplasm.