Given the emergence of P. vivax CQ resistant parasites and the effectiveness of antifolates in malaria vivax treatment together with a strong correlation between mutations in P. vivax dhfr and dhps genes and SP treatment failure,9 the present paper reports an investigation on the pattern of point mutations in pvdhfr and pvdhps genes in Brazilian isolates. The gene discussed is DHFR; the disease is Plasmodium vivax malaria.