TGFB1 and neoplasm: To demonstrate specific T cell activity, we performed in vitro co-culture of splenocytes 7 days after NT or LM-βgal vaccination of WT animals with MCA-OVA as an internal control as our LM-βgal vaccine contains a SIINFEKL peptide, Panc02 tumor cells, FAP KO primary dermal fibroblasts, and FAP KO primary dermal fibroblasts treated with TGFβ to induce activation of β-galactosidase.