The mean percentage methylation in CAD patients was found to be −24.35 ± 3.079 (p < 0.01), 1.924 ± 0.7169 (p < 0.01) and −1.524 ± 0.6976 (p < 0.01) as compared to the mean percentage methylation of −10.03 ± 2.655, 4.369 ± 0.4716 and 1.644 ± 0.7810 in control samples by MS-HRM analysis of STAT1, MHC2 and iNOS, respectively. This evidence concerns the gene NOS2 and coronary artery disorder.