While two fusion-negative cell lines displayed modest responses to NS2028 treatment, with ~30% growth reduction at ~5–10 μM concentration, VCaP cells exhibited a stronger response, with ~45% growth reduction at only ~0.5–1 μM concentration (Fig. 5b), suggesting that the proliferation of ERG-positive PCa cells is more dependent on cGMP signaling. The gene discussed is ERG; the disease is posterior cortical atrophy.