To substantiate that anti-miR-99b and let-7g exert their anti-rotaviral effects specifically by inducing the mTOR pathway leading to negative regulation of autophagy, HT29 cells were transfected with the combination of anti-let-7g and miR-99b (agonist miRNA cocktail) or anti-miR-99b and let-7g (antagonist miRNA cocktail) in presence or absence of a known autophagy inhibitor, 3-MA (3-Methyladenine) followed by RV-SA11 infection. Here, MTOR is linked to infection.