AQP4 and neuromyelitis optica: The E.V.40 mL (elution volume of 40 mL) and E.V.100 mL (elution volume of 100 mL) coming from nSEC separation of DI TNC1 and Sf9 vesicles, respectively, were tested for the binding to AQP4-IgG-containing NMO serum in ELISA sandwich.