In order to characterize the role of CDC42SE1 in the proliferation of skin cancer, we generated three A431 sublines, by infecting A431 cells with lentivirus generated using empty target vector (A431Ctrl), vector expressing CDC42SE1 (A431SE1), and vector expressing CDC42SE1H38A (A431SE1-H38A), and used them for in-vitro analysis. The gene discussed is CDC42SE1; the disease is skin cancer.