Here, we identified an association between SerpinB3 positivity and tumor size in the DEN-HCC rat model as well as a negative correlation between SerpinB3 and caspase-3 mRNA levels in human HCCs, highlighting the contribution of SerpinB3 to the malignant phenotype and tumor progression of HCC. This evidence concerns the gene CASP3 and hepatocellular carcinoma.