We assessed the effect of HRV16 infection (MOI = 5) on IRE1 activity at 3 h, 6 h and 9 h post-infection using specific RT-PCR primers to distinguish between the unspliced (inactive, XBP1u) and spliced (active, XBP1s) forms of the XBP1 transcript. The gene discussed is XBP1; the disease is infection.