GJA3 (Cx46) mRNA levels were lower in MC (dCt = 11.75; SD = 0.66) than in melanoma cell lines A2058 (dCt = 9.85; SD = 0.25), WM983/A (dCt = 8.56; SD = 0.18) and HT199 (dCt = 6.99; SD = 0.22). The gene discussed is GJA3; the disease is melanoma.