GJB1 (Cx32) could not be detected in MC, but it was highly expressed in all three melanoma cell lines: WM983/A (dCt = 6.65; SD = 0.23), HT199 (dCt = 5.25; SD = 0.42) and A2058 (dCt = 4.92; SD = 0.14), though Hs.PT.56a.4848609 TaqMan probe could not detect GJB1 mRNA in HT199 and A2058 cells. The gene discussed is GJB1; the disease is melanoma.