The evaluation of the cytotoxic activity against HT-29 colon and PC-3 prostate cancer cell lines showed that the polycationic dendrimer with four CLB units on the surface (1b) preferentially improves the anticancer activity against the hard to treat PC-3 prostate cancer cell line, blocking the G2/M phase and inducing caspase independent apoptosis. The gene discussed is CLYBL; the disease is prostate carcinoma.