SEMA3A and amyotrophic lateral sclerosis: In order to test the effect of miR-494-3p on its identified target, i.e. motor neuronal Sema3A, we treated HB9-GFP+ mouse MN monocultures with conditioned medium from control or C9-ALS iAstrocytes supplemented with either a scramble miRNA (miR-scr) or a miR-494-3p mimic and then we evaluated the expression level of Sema3A via qRT-PCR.