Most of these studies confirmed the ability of tenovin‐6 to inhibit SIRT1 activity without showing changes in SIRT1 expression.45, 46, 78, 79 In another study, Wei et al showed that the level of SIRT1 was decreased in Omm 1 cells treated with tenovin‐6.80 Our study focuses on assessing synergistic effects between metformin and tenovin‐6 in NSCLC cells irrespective of LKB1 status. This evidence concerns the gene SIRT1 and non-small cell lung carcinoma.