GSK3B and non-small cell lung carcinoma: Interestingly, we found that status of phosphorylated GSK-3β at Tyr 216 were significantly increased in the 3D co-culture with NSCLC and HUVEC cells relative to 2D co-culture with NSCLC and HUVEC cells, but not phosphorylated GSK-3β at Ser 9 (Fig. 3-e and f).