Furthermore, a flow cytometry based Annexin V/ propidium iodide (PI) assay revealed that uremic toxin-induced apoptosis of SH-SY5Y cells was significantly reduced by co-culture with TUDCA-treated CKD-hMSCs, and this effect was reversed by PrPC knockdown (Figure 3E,F). The gene discussed is ANXA5; the disease is chronic kidney disease.