Although both genes had previous connections with neuronal development and splicing [46, 51], this study makes a novel association between alternative splicing and AD with respect to NOS1 and RELN. Since both RELN and NOS1 could exacerbate different mechanisms by which AD arises, it would be interesting for future work to investigate the co-occurrence of variants in these genes and how it impacts neurological function. Here, RELN is linked to Alzheimer disease.