The expression of E‐cadherin decreased but vimentin and N‐cadherin increased in PC‐9/GR cells compared to the PC‐9 cells indicating that PC‐9/GR cells acquired the EMT phenotype.31 As we know, MAPK and PI3K signalling pathways are involved in inducing tumour EMT and play roles in drug resistance.32, 33 In our study, we have verified that TMS increased the sensitivity to gefitinib by reduced EMT by upregulating E‐cadherin and downregulating vimentin and N‐cadherin in PC‐9/GR cells after suppressing MAPK and PI3K/AKT signalling pathways by upregulating miR‐345/miR‐498. This evidence concerns the gene AKT1 and neoplasm.