In order to understand the infection-inducible nature of the zebrafish itln genes at steady state, we quantified the relative mRNA levels of itln1, itln2, itln2-like and itln3 in the liver, spleen, kidney and intestine of unchallenged WT e46 zebrafish by qPCR (Fig. 2A–D). The gene discussed is ITLN2; the disease is infection.