Using U87MG-luc, U87dEGFR and U118MG (grade IV GBM) human cell lines, we examined the effect of progesterone treatments on the expression of Glut1, GAPDH, cytosolic FoxO1 and Phospho-FoxO1 levels, the key players in tumor cell glycolytic metabolism, at 2 and 24 h. The gene discussed is GAPDH; the disease is neoplasm.