MUC13 and infection: We, therefore validated, using quantitative reverse transcription polymerase chain reaction (PCR), upregulation of the clearest example of an infection status marker gene (Fig. 1b), MUC13, in P.berghei-infected primary human hepatocytes, as well as in P.berghei-infected Huh7.5.1, HepG2, and HC04 cells.