As annexin V and PI are used as markers for apoptosis and cell death, respectively, we analysed MCF-7 and MDA-MB-231 cells infected with rMV-BNiP3 supplemented with paclitaxel (30 nM) or H2 (20 μM) prior to infection to ascertain rMV-BNiP3 induced apoptosis using FACS based Annexin V assay. The gene discussed is ANXA5; the disease is infection.