ITIH4 and infection: To understand the early antibody responses to the autologous HIV-1 T/F Env gp120 at higher resolution, variable heavy (VH) and variable light (VL) gene segments were recovered from single IgG+ memory B cells sorted for reactivity with the autologous T/F Env gp120 protein using cryopreserved PBMC collected at 7.5 months after infection (Time Point 2).