The AIF1+, CSF1R+, and CD206+ macrophages were significantly different in the CIS and INV regions (Fig. 8k) and corresponded to higher numbers of pro-inflammatory AIF1+ cells in INV fields (p < 0.0001), while CSF1R+ (p < 0.05), CD206+ (p < 0.01), and iron+ macrophages (p < 0.001) were significantly lower in these same regions. This evidence concerns the gene CSF1R and in situ carcinoma.