In the current study we confirmed that such TAM iron deposits are present in multiple MMTV-PyMT breast cancer models, they are correlated with response to CSF1R breast cancer immunotherapy, and we demonstrated that the spatial infiltration and accumulation of the TAM iron deposits is a factor that is conserved in murine and human breast cancer and differentiates between human breast cancer microenvironments. Here, CSF1R is linked to breast cancer.