The CIS and INV fields stained for Prussian blue iron+ HLMs (Fig. 8a,b) were re-stained for inflammatory M1-like macrophages (Fig. 8c,d; AIF1), anti-inflammatory M2-like macrophages (Fig. 8e,f; CD206), and the CSF1R receptor (Fig. 8g,h) in order to determine the co-positivity of iron with these markers (Fig. 8i,j). The gene discussed is AIF1; the disease is in situ carcinoma.