Additionally, the calculation of the fraction of the iron+AIF1+ and iron+CD206+ populations with CSF1R indicated that significantly more iron+CSF1R+AIF1+ (p < 0.001) and iron+CSF1R+CD206+ (p < 0.001) macrophages were present in CIS fields and iron+CSF1R+AIF1+CD206+ populations were largely the same in the CIS and INV microenvironments (p > 0.05). This evidence concerns the gene MRC1 and in situ carcinoma.