Controlling for the primary inhibitory effect on the accumulation of iron+ populations, fields containing the iron+ TAMs in control tumors and fields still containing iron deposits following BLZ945 treatment were identified in the registered tumor cross-sections in order to quantify the co-positivity of the iron+ TAMs as a function of M1-like, M2-like, and CSF1R status in these localized regions. Here, CSF1R is linked to neoplasm.