This analysis confirmed that both tumour cells and normal ISCs expressing the Notch1 receptor are undifferentiated, as they show downregulation of differentiation markers, such as Mucin2 (Muc2), the Regenerating Family Member 4, Reg423, Chromogranin A24 (Chga), Doublecortin Like Kinase 1 (Dclk125), Alpi and Membrane Alanyl Aminopeptidase (Anpep26) both enterocyte markers, while they express high levels of reported ISCs markers, including Olfm427, Lrig128,29, Smoc230, Hopx and Aldh1b1 (Aldehyde dehydrogenase 1b131) (Fig. 3c). The gene discussed is REG4; the disease is neoplasm.