We next assessed the cellular composition of Notch-derived clones and found that Notch1+ tumour cells can generate the repertoire of different cell types found within intestinal adenomas, as Notch1 lineages contain both proliferative (marked by Proliferating Cell Nuclear Antigen, PCNA in Fig. 2f) and differentiated tumour cells (marked by Lyz1, Muc2 and ChgA in Fig. 2f). This evidence concerns the gene CHGA and neoplasm.