To assess multipotency of the Notch1+ colon cancer cells, we analysed their clonal progeny (EpCAM+) and found that they express both proliferation (Ki67+) and differentiation markers (i.e. Mucin2 shown in Fig. 5c), demonstrating that Notch1-expressing cells in colon tumours have multilineage differentiation potential and present the same properties of Notch1+ intestinal adenoma cells. Here, NOTCH1 is linked to colonic neoplasm.