Next, we defined the genome-wide transcriptional signature of Notch1+ tumour cells and normal ISCs by performing Affymetrix analyses of FAC-sorted GFP+ and GFP- tumour and normal crypt cells derived from N1-Cre/R26mTmG/Apc and N1-Cre/R26mTmG mice, respectively. This evidence concerns the gene NOTCH1 and neoplasm.