Co-culture of human colorectal carcinoma HCT116 cells with human colon fibroblast CCD-18Co cells in 3D spheroid microtumor structures activated CCD-18Co cells to CAFs with a gene signature similar to patient CAFs, among which GPR68 was one of the most highly up-regulated genes during this process. This evidence concerns the gene GPR68 and colorectal carcinoma.