Using bioinformatic methods, we further identified putative targets of these microRNAs, which were downregulated in bronchial epithelial cells from both asthma and COPD: miR-10a-5p might suppress BCL2, FGFR3, FOXO3, PDE4A, PDE4C, and PDE7A; miR-146a-5p might suppress BCL2, INSR, PDE4D, PDE7A, PDE7B, and PDE11A. The putative targets were further validated with primary bronchial epithelial cells, showing significantly decreased levels of FOXO3 and PDE7A in AHBE and CHBE than in NHBE, as well as significantly decreased level of BCL2 in CHBE than in NHBE. Here, PDE4D is linked to chronic obstructive pulmonary disease.