To determine the effect of the microenvironment on the expression of glioblastoma stem cell markers, we examined the mRNA expression of CD133 and OCT4 in the cells cultured in the 3D assay and cells cultured as 2D (3D to 2D) and neurospheres (3D to neurosphere) following extraction from the 3D assay (Fig. 1). Here, PROM1 is linked to glioblastoma.