In this study, in order to investigate the molecular etiology of non‐syndromic deafness patients from Shanxi Province, we have performed mutation analysis of three common deafness‐related genes (GJB2, SLC26A4, and mtDNA 12S rRNA) in 1,201 patients with non‐syndromic deafness in Shanxi Province, which has a high incidence of HL. Here, GJB2 is linked to deafness.