In the checkpoint-resistant breast TSA model (mouse), it was observed that single high dose (20 or 30 Gy) of RT did not induce abscopal effects when used along with either anti-CTLA-4 or anti-PD-1 while a short fractionated course (8 Gy*3) induced an abscopal systemic immune response when given in conjunction with anti-CTLA-4 leading to prolonged/sustained tumor regression. This evidence concerns the gene CTLA4 and neoplasm.