Treatment of mature sterile VEC multilayers with a low or high dose of the agonists or the DPBS vehicle control for 2 h before apical infection with ZIKV produced a modest reduction in ZIKV titers that was dose dependent when low dose LPS (TLR4) or FLAG (TLR 5) were compared to high dose treatments (Supplementary Figure 4). This evidence concerns the gene TLR4 and infection.