To determine how Dnmt3aR878H/+ CH progresses to MPD and AML with acquisition of Npm1cA mutation, we examined the BM stem and progenitor cell compartment of primary and secondary recipient mice transplanted with Dnmt3aR878H/+Npm1cA/+ cells (representative gating shown in Fig. 5a). This evidence concerns the gene C4B and acute myeloid leukemia.