(H, I): ZNF326 was nuclear positive (nearly 50%, ++), while HDAC7 (>50% positive cells, ++) was perinuclear cytoplasm positive in anaplastic astrocytoma (grade III, Magnification 400×), (K, L): ZNF326 and HDAC7 were strongly expressed (nucleus and cytoplasm, respectively, >75% positive cells, +++) in glioblastoma with grade IV (Magnification 400×). Here, ZNF326 is linked to anaplastic astrocytoma.