In this study, we analyzed FN and αvβ3 expression levels via immunohistochemistry and western blotting and examined correlation of the individual expression as well as co-expression with the clinicopathological features, disease-free survival (DFS), and overall survival (OS) of patients with osteosarcoma to identify the potential clinicopathological and prognostic values of these factors in osteosarcoma. The gene discussed is FN1; the disease is osteosarcoma.