To further verify the involvement of ADAM17, we treated the cells with TAPI-1, a specific inhibitor of ADAM17 (Raikwar et al., 2014), prior to PCN033 infection, and found that the time-dependent phosphorylation of EGFR induced by PCN033 was completely blocked by TAPI-1 (Supplementary Figure 3B). This evidence concerns the gene ADAM17 and infection.