Regarding CD4+ T-cells functional profile after PPD stimulation, cells producing only IFN-γ were significantly lower compared to cells producing only TNF-α+ or IFN-γ+/TNF-α+ simultaneously in active TB patients (p < 0.0001 for IFN-γ+ vs. TNF-α+ and p < 0.0001 for IFN-γ+ vs. IFN-γ+/TNF-α+) and LTBI individuals (p = 0.0001 for IFN-γ+ vs. IFN-γ+/TNF-α+). The gene discussed is CD4; the disease is tuberculosis.