In this context, we found that CD27− and/or CCR4+ expression within: (i) TNF-α+CD4+; (ii) IFN-γ+TNF-α+CD4+ T-cells, and (iii) IFNγ+and/orTNF-α+CD4+ T-cells, as well as CD27 MFI ratio measured in these functional populations, were increased in active TB patients in comparison with LTBI individuals. Here, TNF is linked to tuberculosis.