As shown in Fig. 3b and c, after treatment with DNA methyltransferase inhibitor 5-Aza-dC, the expression levels of ZNF667-AS1 and ZNF667 were all increased in laryngeal cancer cell lines, indicating the important role of DNA methylation in silencing the expression of ZNF667-AS1 and ZNF667. This evidence concerns the gene ZNF667 and laryngeal carcinoma.