In this study, as nucleus-localized MALAT1 does not regulate EGFR mRNA and the protein synthesis machine localizes in cytoplasm, we assumed that a mediator manipulating the protein synthesis of EGFR is transcriptionally regulated by MALAT1. Transcriptomic sequencing and subsequent studies confirmed the role of KTN1 in mediating the regulation of EGFR protein expression by MALAT1. Thus, the different indirect regulatory mechanisms proposed in our and other studies may suggest the exact mechanism about how MALAT1 regulates EGFR is cancer-specific. This evidence concerns the gene MALAT1 and cancer.