EGFR and skin squamous cell carcinoma: As the A431 cell line is featured in EGFR gene amplification [14], the strong tumor-suppressive effect by MALAT1 depletion suggested that EGFR might be regulated by MALAT1. To test this hypothesis, IHC and western blot assays were firstly performed and revealed that EGFR protein is higher expressed in cSCC tumors than in normal skin tissues (Fig. 3a, b), which coordinated with MALAT1 expression in cSCC (Fig. 1d).