Our findings in this study show the presence of both CD103+ and CD8a+ DC populations in brains and TDLNs of brain tumor-bearing mice, and total DC populations with higher proportions of CD103+/CD8a+ cells have activated OT-I T cells in vitro and induced OT-I T cell proliferation in vivo, showing that CD103+/CD8a+ DC populations in the brain have roles in antigen-presenting and cross-priming effects, as observed in peripheral tumors. This evidence concerns the gene CD8A and brain neoplasm.