Then we assessed a range of markers related to tumour cell apoptosis and proliferation via IHC, the results of which indicated that the numbers of cleaved caspase 3-positive and transferase-mediated deoxyuridine triphosphate-biotin nick end labelling (TUNEL)-positive tumour cells were significantly higher in the two NSD2-KD groups than in the control group, although there were no significant differences in Ki67 expression among the groups (Fig. 3d). This evidence concerns the gene CASP3 and neoplasm.