Although the overall difference among the groups did not reach significance for a distinct change in PBMC IFNG mRNA expression (P = 0.24, Table 4), there was a trend for PBMC IFNG mRNA expression to decrease in the CSO group (β-coefficient ± SD: −0.17 ± 0.1, P = 0.087, Fig. 1), but not in the other study groups (P > 0.50 for both FF and LF) in relation to the control group. This evidence concerns the gene IFNG and craniosynostosis.