To further elucidate the anti-migratory and anti-invasive functions of SHROOM2, a spontaneous lymph node metastasis mouse model was established by injecting SHROOM2 knockdown NPC cells and control cells into the left hind footpad of immune-deficient nude mice, which resulting in metastasis to the popliteal lymph node. This evidence concerns the gene SHROOM2 and nasopharyngeal carcinoma.