To investigate the effect of PRDM16 on cell growth and mobility in lung adenocarcinoma, we overexpressed PRDM16 in H1299, Calu-1 and A549 cells (Fig. 2a) or knocked down its expression in H1299 and Calu-1 cells (Fig. 2b). Here, PRDM16 is linked to lung adenocarcinoma.