Taken together, our proteomic analysis of OMVs and biological validation suggest that Tobramycin may improve lung function in CF patients infected with P. aeruginosa in part by reducing AprA in OMVs, an effect that would mitigate the adverse effect of OMVs on Phe508del CFTR Cl- secretion, which is essential for bacterial clearance from the lungs. Here, CFTR is linked to cystic fibrosis.