Therefore, the aim of this study were to investigate the effects of supplementing different complexes of CrHis and CrPic supplementation along with biotin on the insulin sensitivity and also to evaluate the anti‐obesity properties of these supplements through their action of mechanism by looking at the changes in biomarkers such as PPAR‐γ, IRS‐1, GLUTs, NF‐κB proteins, metabolic parameters, and tissue histopathological changes in rats fed HFD. This evidence concerns the gene PPARG and obesity due to melanocortin 4 receptor deficiency.