Studies have documented that leptin induced the secretion of tumor necrosis factor and interleukins-6 and also increases the accumulation of reactive oxygen species.25 Hypercholesterolemia also causes endothelial cell injury that is contributed with up-regulation of some inflammatory factors such as NF-κB, interleukin-6 and TNF-α.26 The results of this study were in a parallel with Wang et al study who examined the effect of dietary bLF on antioxidant and performance status in piglet. The gene discussed is TNF; the disease is familial hypercholesterolemia.